Category: SecureSeal

Transcriptome Profiling

RNA sequential probing of targets (SPOTs) is a newly developed method for mRNAs profiling, based on sequential Fluorescence In Situ Hybridization (seqFISH). SeqFISH is a technology that enables the identification of many RNA transcripts directly in single cells preserving spatial context. To demonstrate the method “mRNA, extracted from mouse cells, was captured on a locked nucleic acid … Continued

Conserve Reagents with Miniature Western Blot Reaction Chambers

SecureSeal Hybridization Chambers were originally developed to perform hybridizations on specimens affixed to glass slides or to perform microarray incubations with limited volumes (SecureSeal References). The peel- and- stick adhesive chambers can be created in various sizes to accommodate different samples, including partial membrane blots from protein mini-gels (shown here).  Recently we have investigated the … Continued

Protein Microarrays on the Brink?

Microarray technology was first exploited with genomic analysis of DNA sequences.  This technology still forms a robust platform for biomarker discovery and comparative genomics. However, while genomics sheds light on the biological potential, a recent review in Science Signaling  points out that  “protein does the heavy lifting in the cell, and neither DNA nor RNA says much … Continued