Category: Seals and chambers

Transcriptome Profiling

RNA sequential probing of targets (SPOTs) is a newly developed method for mRNAs profiling, based on sequential Fluorescence In Situ Hybridization (seqFISH). SeqFISH is a technology that enables the identification of many RNA transcripts directly in single cells preserving spatial context. To demonstrate the method “mRNA, extracted from mouse cells, was captured on a locked nucleic acid … Continued

Flow chambers with precise control of environmental conditions for single-cell assays

A simple fluidic design integrates directly into standard cell culture and biochemical assays as a part of new experimental methods published recently. The fluidic design used single channel or T-shaped flow chambers and a syringe pump to effectively culture and assay cells in a continuous gradient of conditions. These methods simultaneously expand experimental capability and … Continued

Enabling Cell-based Assays and Translation to Personalized Medicine

Cell-based assays have expanded in use, and reduced in sample requirements over the past decade, driven by technologies that have improved the culture and monitoring of cell functions on a miniature scale. One of our partners, Microstem Inc., based in San Diego, CA, is an example of an early stage reagent development company that approached … Continued

Drug Delivery as Important as the Drug

It is fascinating to see how our customers utilize the simple and elegant designs of our chambers to facilitate their research. A recent paper in PNAS by GW Ashley et al. illustrates this point quite well. Our CoverWell perfusion chambers were originally designed for live cell imaging, with medical grade silicone adhered to a plastic ‘window’ … Continued

Conserve Reagents with Miniature Western Blot Reaction Chambers

SecureSeal Hybridization Chambers were originally developed to perform hybridizations on specimens affixed to glass slides or to perform microarray incubations with limited volumes (SecureSeal References). The peel- and- stick adhesive chambers can be created in various sizes to accommodate different samples, including partial membrane blots from protein mini-gels (shown here).  Recently we have investigated the … Continued

ProPlate® Chambers Minimize Microarray Variation

Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued

Print Macroarrays with ProPlate

ProPlate® Chambers for Macroarray Printing Many laboratories desire the benefits of utilizing microarray technology in their field of interest but do not have access to microarray printing equipment or cannot justify the expense of dedicated print runs for lower throughput experiments.  In many cases where highly multiplexed assays are not a necessity or during early-phase … Continued

Cancer Research: Universal Drug Target for Cancer?

The founders of Grace Bio-Labs initiated their scientific careers with cancer research, and we continue to follow this literature with great interest. An interesting article in NY Times recently reported on a new strategy in cancer therapies being pursued by several major pharmaceutical companies.It has long been known that de-regulation of p53 protein is a key factor … Continued

Protein Microarrays: Investment Required

As scientists we are trained to be independent thinkers, and to value the expertise gained on the learning curve of new technologies. When it comes to microarrays, however, outsourcing or collaborating with an expert can accelerate the development process.“There is a serious investment required in assay development for microarrays,” says Stacey Clarken, VP of Commercial … Continued

Design Considerations for Microfluidic Reaction Chambers

Microfluidic reaction chambers are often designed for a hybridization or incubation step in molecular analysis to contain molecular interactions in a controlled environment. An example we are very familiar with is in situ hybridization, in which cells or tissue is placed on a slide and a label or antibody is placed in solution on the … Continued