Category: ProPlate

Construction and Analysis of Glycan Microarrays

Protein glycosylation is a complex post-translational modification (PTM) that provides great proteomic diversity. It involves the attachment of sugars at specific amino acid residues. Asn (N-linked) or Ser/Thr (O-linked) being the residues most frequently glycosylated. Glycosylation is critical for physiological and pathological cellular functions and affects the bioavailability, efficacy, stability, and solubility of therapeutic glycoproteins. … Continued

Rapid Arsenite Assay developed with ProPlate® Multi-Well Chambers

It is always fascinating to learn how our customers take advantage of the versatility of Grace Bio-Labs’ ProPlate® Multi-Well Chambers. Initially designed to take full advantage of the multiplexing and high-throughput potential of microarray technology, ProPlates feature a compressible gasket applied on a polycarbonate upper chamber. Affixed on a glass slide they create the leak-proof … Continued

Antigen Array For Serological Diagnosis For Severe Equine Asthma

Grace BioLabs Oncyte® NOVA slides and ProPlates® were used to elucidate previously unidentified allergens involved in the potential diagnosis of severe equine asthma (sEA). The microarray contained a complex matrix of fungi, bacteria, pollen, arthropods extracts, and proteins. It was tested against serum obtained from a mixed group of 138 horses living in varying environments. … Continued

Plasmonic gold chip for diagnosis of myocardial infarction

In a recent publication, Wei Xu et al. proposed a novel approach for the diagnosis and monitoring of markers of myocardial infarction. The method is based on on-chip microarray analysis of serum biomarkers (e.g., cardiac troponin I) and when coupled with NIR-FE (NearInfraRed – fluorescent enhancement) showed a considerable increase in sensitivity and specificity over … Continued

ProPlate multi-well chambers utilized to generate a synthetic hydrogel array for embryonic stem cell expansion and vascular toxicity studies.

Introduction Matrigel is a natural extracellular matrix (ECM) secreted by Englebreth-Holm Swam mouse sarcoma. It has numerous applications in 2D and 3D cell culture for its ability to mimic in vivo environments. However, its complex and highly variable composition can negatively affect experimental reproducibility and accuracy. Furthermore, components naturally occurring in Matrigel can interfere with … Continued

Enabling Cell-based Assays and Translation to Personalized Medicine

Cell-based assays have expanded in use, and reduced in sample requirements over the past decade, driven by technologies that have improved the culture and monitoring of cell functions on a miniature scale. One of our partners, Microstem Inc., based in San Diego, CA, is an example of an early stage reagent development company that approached … Continued

ProPlate® Chambers Minimize Microarray Variation

Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued

Print Macroarrays with ProPlate

ProPlate® Chambers for Macroarray Printing Many laboratories desire the benefits of utilizing microarray technology in their field of interest but do not have access to microarray printing equipment or cannot justify the expense of dedicated print runs for lower throughput experiments.  In many cases where highly multiplexed assays are not a necessity or during early-phase … Continued

Protein Microarrays on the Brink?

Microarray technology was first exploited with genomic analysis of DNA sequences.  This technology still forms a robust platform for biomarker discovery and comparative genomics. However, while genomics sheds light on the biological potential, a recent review in Science Signaling  points out that  “protein does the heavy lifting in the cell, and neither DNA nor RNA says much … Continued