Category: Product Applications

Transcriptome Profiling

RNA sequential probing of targets (SPOTs) is a newly developed method for mRNAs profiling, based on sequential Fluorescence In Situ Hybridization (seqFISH). SeqFISH is a technology that enables the identification of many RNA transcripts directly in single cells preserving spatial context. To demonstrate the method “mRNA, extracted from mouse cells, was captured on a locked nucleic acid … Continued

Vitrification Cassette For The Cryopreservation of Cryptosporidium Parvum Oocysts

Scientists at Tufts University and Harvard Medical School have developed a method to cryopreserve infectious Cryptosporidium oocysts. The results were published in Scientific Reports. Cryptosporidium is a microscopic parasite that causes the diarrheal disease cryptosporidiosis. While this parasite can be spread in many different ways water is the main source of transmission. Infants and immunocompromised adults are most at … Continued

Electromechanical stimulation of cardiomyocytes derived from iPSCs

Human-induced pluripotent stem cell (iPSC) technology has taken the center stage in regenerative medicine research thanks to its enormous potential for personalized treatment and drug screening applications. Reprogramming somatic cells to generate iPSCs (induced pluripotent stem cells) is not only an easier and faster way to obtain stem cells, but is also devoid of the ethical … Continued

Flow chambers with precise control of environmental conditions for single-cell assays

A simple fluidic design integrates directly into standard cell culture and biochemical assays as a part of new experimental methods published recently. The fluidic design used single channel or T-shaped flow chambers and a syringe pump to effectively culture and assay cells in a continuous gradient of conditions. These methods simultaneously expand experimental capability and … Continued

RPPA Analysis of Mild Traumatic Brain Injuries Biomarkers

A growing number of studies indicate that repeated mild traumatic brain injuries (RmTBI) are associated with significant and lasting neurological alterations and an increased risk of developing neurodegenerative diseases. Advanced Magnetic Resonance Imaging and blood-based protein analysis not only can provide a reliable method for the clinical assessment and treatment of RmTBI but also valuable … Continued

Epitope Mapping Using Peptide Microarray

Epitope mapping allows the identification of polyclonal antibodies produced as a response to cytotoxins, allergens, neuronal or inflammatory conditions and, in autoimmune diseases to autoantigens. Routine assays for autoimmune response profiling are generally performed by ELISAs and fluorescence immunoassays. Individual assays are performed in microtiter plates, with each well representing a single antigen. Peptide microarrays provide … Continued

Applications of Protein Microarrays for Biomarker Discovery.

In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated. An antigen microarray assay was performed with ten different antigens spotted on ONCYTE® nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued

Multiplexed ONCYTE Protein microarrays Used for Infectious Disease Immunogen Discovery

First, in an interesting Shistotosomiasis study, Gaze, et al, employ protein microarrays in a longitudinal immunomics study for vaccine antigen discovery (1). Schistosomiasis is a pervasive disease affecting 200 million people a year and traditional drug treatments are ineffective due to re-infection, drug expense, and parasite drug resistance. Non-sterile immunity does develop over long periods of … Continued

Beyond In Situ Hybridization; Novel Multiplex Immunoassay Applications of HybriWell™ Microslide Chambers

One of the best aspects of working with Grace Bio-Labs and the in-situ hybridization and chambering devices is the opportunity to learn some novel applications which are developed by the users of these tools. Sometimes our customers can teach us a few things about applications for our products!As recently as this summer, a very interesting … Continued

A Comprehensive Technology Survey of Protein Microarray Platforms, Techniques and Detection Methods

A comprehensive assessment of multiplex immunoassay platforms and detection methods is summarized in an interesting 2011 article by Chandra, et al, in Expert Review Proteomics (1). The article, “Protein Microarrays and Novel Detection Platforms” provides interesting information for several reasons.First, it presents an excellent description of the emerging discovery and clinical need for high throughput … Continued

Sensitive and Accurate Multi-Color RPPA Using Grace Bio-Labs ArrayCAM Protein Microarray System

Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued

Improved Classification of Influenza Infection, Future Susceptibility and Pandemic Epidmiology using ONCYTE Porous Nitrocellulose Film Microarrays

An interesting paper furthering the expansion of infectious disease serological applications using ONCYTE porous nitrocellulose film slides was described by researchers out of the Netherlands for classifying influenza infections and susceptibility to future infections. Microarrays printed on ONCYTE AVID film slides used purified recombinant influenza virus hemagglutinin antigens (HA1) from various subtypes (H1, H2, H3, … Continued

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