Category: PATH non-porous nitrocellulose

PATH® SARS-CoV2 Antigen Microarray

SARS-CoV2 seroprevalence data indicates that asymptomatic transmission is largely underreported. It is also known to play an important role in disease transmission among the general population.   Asymptomatic individuals, defined as persons with no clinical symptoms, no radiological sign of the disease in the lungs, and a positive SARS-CoV2 nucleic acid test result, shed viral particles … Continued

Construction and Analysis of Glycan Microarrays

Protein glycosylation is a complex post-translational modification (PTM) that provides great proteomic diversity. It involves the attachment of sugars at specific amino acid residues. Asn (N-linked) or Ser/Thr (O-linked) being the residues most frequently glycosylated. Glycosylation is critical for physiological and pathological cellular functions and affects the bioavailability, efficacy, stability, and solubility of therapeutic glycoproteins. … Continued

SARS-CoV-2 Proteome Microarray for IgG and IgM Response Profiling

Convalescent plasma is a potential therapeutic option for patients with coronavirus disease. The therapy is based on the administration of plasma containing antibodies against SARS-CoV-2 to help patients boost their responses to the coronavirus. Additionally, convalescent plasma can also be used for passive immunization with the potential to prevent viral infection. In both applications, determining … Continued

Protein glycosylation profiling with PATH ® microarray slides

Protein glycosylation analysis presents some inherent challenges due to the complexity of glycans. Determination of the mass, linkage, Isomerization and anomerization state of a protein require a combination of approaches for which relatively large amounts of proteins are needed. Such amounts are not always available when analyzing precious clinical specimens, making it difficult to extend … Continued

Antibody Validation Assay with PATH® Protein Microarray Substrate

The analysis of antibody interactions to 19,500 recombinant full-length human proteins featured on HuProt™ Human Proteome Microarray provides a robust and rapid method to evaluate antibodies’ quality. Introduction Antibodies are the most commonly used research tools. Applied in basic research as well as in clinical and diagnostic medicine, their quality, consistency, and availability are fundamental … Continued

Epitope Mapping Using Peptide Microarray

Epitope mapping allows the identification of polyclonal antibodies produced as a response to cytotoxins, allergens, neuronal or inflammatory conditions and, in autoimmune diseases to autoantigens. Routine assays for autoimmune response profiling are generally performed by ELISAs and fluorescence immunoassays. Individual assays are performed in microtiter plates, with each well representing a single antigen. Peptide microarrays provide … Continued