Category: ONCYTE nitrocellulose film slides

Construction and Analysis of Glycan Microarrays

Protein glycosylation is a complex post-translational modification (PTM) that provides great proteomic diversity. It involves the attachment of sugars at specific amino acid residues. Asn (N-linked) or Ser/Thr (O-linked) being the residues most frequently glycosylated. Glycosylation is critical for physiological and pathological cellular functions and affects the bioavailability, efficacy, stability, and solubility of therapeutic glycoproteins. … Continued

Microarray Technology for The Analysis Of The Secretome

A newly published editorial offers a comprehensive overview of the different approaches applied to the study the secretome, the subset of proteins secreted by a particular organism. Scientists are increasingly targeting this complex and homogenous collection of proteins with the hope to uncover new biochemical pathways and to identify new therapeutic and diagnostic targets. While … Continued

Coronavirus Antigen Microarray for SARS-CoV-2 Antibodies Analysis

The diagnosis of coronavirus infection relies on RT-PCR analysis of upper respiratory and nasopharyngeal specimens. The limited testing capacity and the wide variability of patients’ viral load have so far provided a narrow view of the pervasiveness of the infection among the population. A recently developed coronavirus antigen microarray represents a promising diagnostic and epidemiologic … Continued

ONCYTE® Protein Microarrays to study B cell response to avian influenza virus

Avian influenza viruses represent a major public health concern, and worldwide outbreaks raise the risk of a potential pandemic. Sero-epidemiological studies typically rely on data obtained through hemagglutination inhibition assays (HAI). HAI has been shown to display low sensitivity towards avian influenza antibodies resulting in difficult and inconsistent results interpretations. Furthermore, a previous protein array-based … Continued

Cancer Cell Line Encyclopedia Expanded to Include RPPA Analysis Data

The Cancer Cell Line Encyclopedia is a collaborative project started in 2008 with the goal to characterize, from a genetic and pharmacological standpoint, a large panel of human cancer models. The idea was to develop data linking genetic profiles to pharmacological outcomes that can translate into personalized therapeutic regimens and accelerate cancer research. The dataset … Continued

RPPA Analysis of Mild Traumatic Brain Injuries Biomarkers

A growing number of studies indicate that repeated mild traumatic brain injuries (RmTBI) are associated with significant and lasting neurological alterations and an increased risk of developing neurodegenerative diseases. Advanced Magnetic Resonance Imaging and blood-based protein analysis not only can provide a reliable method for the clinical assessment and treatment of RmTBI but also valuable … Continued

Adaptive Immune Response Captured by Proteome Microarray using Oncyte Slides

Profiles of immune system antibodies provide a comprehensive viewpoint to develop a broad-spectrum pneumonia and meningitis vaccine. Vaccine Development using Protein Microarray A vaccine trains an individual’s immune system to recognize the disease-causing bug as a threat. Robust vaccination creates an immune response to any variant of the bug the individual might encounter throughout life. … Continued

RPPA Reveals Altered Phosphorylation Profile in Plasmodium-infected Hepatocytes

Introduction Plasmodium parasites inflict widespread global disease, which in the case of malaria is caused by the intraerythrocytic form of the parasite.  The parasite infects the host by traveling to the liver where it intracellularly develops and affects hepatocyte cells, initiating a range of responses including autophagy and apoptosis.  Different hosts, and even different hepatocyte … Continued

RPPA Metabolic Profiling Identifies Glioblastoma Subtypes That Correlate With Prognostic Outcome.

Introduction Glioblastoma (GMB) is the most aggressive type of brain cancer and represents 15% of all forms diagnosed.  Symptoms are initially non-specific but progress rapidly.  GMB’s exhibit aggressive growth, limited response to therapeutic intervention and high instance of reoccurrence.  As the survival rate for patients is 12- 15 months after diagnosis, early detection is critical. … Continued

Malaria vaccine development: a microarray to identify new target proteins

Malaria is a life-threatening disease caused by Plasmodium parasites transmitted to people through the bites of infected female Anopheles mosquitoes. In 2017, it was estimated that nearly half of the world population was at risk of contracting the disease, with the highest number of cases and deaths reported in sub-Saharan Africa. In the same year … Continued

Quantitative analysis of lectin-bacteria interactions of Haemophilus influenza strains.

A panel of biotin-label lectins is used to interrogate bacterial extract and purified lipopolysaccharides arrayed on a 16 pad ONCYTE Supernova microarray slide. Bacterial surfaces are decorated with short chain lipopolysaccharides (LOSs). These molecules mediate bacterial mobility and cell adhesion. They are also involved in the host immune system evasion and modulation. In fact, one … Continued

Applications of Protein Microarrays for Biomarker Discovery.

In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated.  An antigen microarray assay was performed with ten different antigens spotted on ONCYTE®  nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued