Plasmodium Falciparum Proteome-Wide Microarray

Humoral immune response profiling is a valuable tool for the identification of potential targets for vaccine development. Immunogenicity studies in clinical trials are usually performed by ELISA assay. However, technical constraints limit the number of antigens that can be simultaneously analyzed.  These limitations can be overcome by employing microarray technology, where antigenic reactivity can be … Continued

ONCYTE® Protein Microarrays to study B cell response to avian influenza virus

Avian influenza viruses represent a major public health concern, and worldwide outbreaks raise the risk of a potential pandemic. Sero-epidemiological studies typically rely on data obtained through hemagglutination inhibition assays (HAI). HAI has been shown to display low sensitivity towards avian influenza antibodies resulting in difficult and inconsistent results interpretations. Furthermore, a previous protein array-based … Continued

RPPA Analysis of Mild Traumatic Brain Injuries Biomarkers

A growing number of studies indicate that repeated mild traumatic brain injuries (RmTBI) are associated with significant and lasting neurological alterations and an increased risk of developing neurodegenerative diseases. Advanced Magnetic Resonance Imaging and blood-based protein analysis not only can provide a reliable method for the clinical assessment and treatment of RmTBI but also valuable … Continued

The Trafficking of wheat prolamins in rice endosperm

Wheat is the most important crop in Europe due in part to high relative crop yields over a range of environments. “(Wheats) success as a food crop is also due to the unique properties which form the gluten fraction… It (is) necessary to understand the pathways and mechanisms which determine the synthesis, processing, trafficking and … Continued

Applications of Protein Microarrays for Biomarker Discovery.

In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated.  An antigen microarray assay was performed with ten different antigens spotted on ONCYTE®  nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued

Improved Classification of Influenza Infection, Future Susceptibility and Pandemic Epidmiology using ONCYTE Porous Nitrocellulose Film Microarrays

An interesting paper furthering the expansion of infectious disease serological applications using ONCYTE porous nitrocellulose film slides was described by researchers out of the Netherlands for classifying influenza infections and susceptibility to future infections. Microarrays printed on ONCYTE AVID film slides used purified recombinant influenza virus hemagglutinin antigens (HA1) from various subtypes (H1, H2, H3, … Continued

Quantum Nanocrystal (QNC) Detection and ONCYTE AVID Porous Nitrocellulose Results in Improved RPPA Quantitation and Dynamic Range

ONCYTE porous nitrocellulose (PNC) film slides are a 3-D substrate very well suited for protein microarrays due to its high binding capacity and ability to retain proteins’ conformation and antigenic sites. PNC slides have proven extremely valuable for Reverse-Phase Protein Arrays (RPPAs) in the field of cancer research.In recent years, interest in further improving the … Continued

Review of Mujawar et al Analytical Chemistry – Distribution of Biomolecules in Porous Nitrocellulose Films

An interesting paper in (Analytical Chemistry) describes the use of confocal fluorescence section imaging to analyze protein distribution in microarray spots on nitrocellulose film slides. By scanning and imaging through the Z dimension of a printed spot of fluor labeled protein, a 3-dimensional quantitation of fluorophore density in the films was obtained, showing that commercial … Continued

Sensitive Detection of Total Protein on RPPA using Fast Green

Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued

View Your Proteins in 3-D!

Protein microarray technology has progressed significantly and, although new microarray surface chemistries continue to be developed and marketed, porous nitrocellulose has remained the predominant immobilization surface for protein microarray applications due to its many functional advantages.  The 3-dimensional structure of nitrocellulose film slides offers a considerably higher surface area for protein binding compared to conventional … Continued

Print Macroarrays with ProPlate

ProPlate® Chambers for Macroarray Printing Many laboratories desire the benefits of utilizing microarray technology in their field of interest but do not have access to microarray printing equipment or cannot justify the expense of dedicated print runs for lower throughput experiments.  In many cases where highly multiplexed assays are not a necessity or during early-phase … Continued