Category: HybriWell

Novel Biosensors To Study Kinase Activation In Plants

HybriWell™ hybridization chambers are the ideal tool for conserving precious or costly reagents in imaging experiments. HybriWells™ are inexpensive, disposable devices that create a chamber whose ports allow liquid exchange via microfluidic passive pumping [2], as demonstrated in this video: Seitz et, al. recently applied the method to develop and test fluorescence-based genetically-encoded biosensors to study … Continued

Flow chambers with precise control of environmental conditions for single-cell assays

A simple fluidic design integrates directly into standard cell culture and biochemical assays as a part of new experimental methods published recently. The fluidic design used single channel or T-shaped flow chambers and a syringe pump to effectively culture and assay cells in a continuous gradient of conditions. These methods simultaneously expand experimental capability and … Continued

Conserve Reagents with Miniature Western Blot Reaction Chambers

SecureSeal Hybridization Chambers were originally developed to perform hybridizations on specimens affixed to glass slides or to perform microarray incubations with limited volumes (SecureSeal References). The peel- and- stick adhesive chambers can be created in various sizes to accommodate different samples, including partial membrane blots from protein mini-gels (shown here).  Recently we have investigated the … Continued

Protein Microarrays on the Brink?

Microarray technology was first exploited with genomic analysis of DNA sequences.  This technology still forms a robust platform for biomarker discovery and comparative genomics. However, while genomics sheds light on the biological potential, a recent review in Science Signaling  points out that  “protein does the heavy lifting in the cell, and neither DNA nor RNA says much … Continued