Category: Featured Research

Transcriptome Profiling

RNA sequential probing of targets (SPOTs) is a newly developed method for mRNAs profiling, based on sequential Fluorescence In Situ Hybridization (seqFISH). SeqFISH is a technology that enables the identification of many RNA transcripts directly in single cells preserving spatial context. To demonstrate the method “mRNA, extracted from mouse cells, was captured on a locked nucleic acid … Continued

Confocal Fluorescence Microscopy of CRISPR-generated G6PD Knockout mouse liver cells (TIB-73)

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas-9 knockout of glucose 6-phosphate dehydrogenase (G6PD) The first surviving G6PD- knockout somatic cell line was generated with CRISPR-Cas-9 technology to support subcellular localization studies. G6PD,  glucose 6-phosphate dehydrogenase, is the rate-limiting enzyme in the pentose phosphate pathway. G6PD is involved in oxidative stress, apoptosis, angiogenesis and cancer … Continued

Sensitive and Accurate Multi-Color RPPA Using Grace Bio-Labs ArrayCAM Protein Microarray System

Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued

Validation of a Novel One-Step Tissue Fixation Chemistry That Preserves Phosphoproteins and Histomorphology

  George Mason University Poster Presentation for the National Cancer Institute’s 15th Annual IMAT Principle Investigators Meeting, November 13-15, 2014. Unmet need: A significant and underappreciated issue is the fact that excised tissue is alive and reacting to ex vivo stress [1]. During this “cold ischemia time” cells within the tissue react and adapt to the absence … Continued

Multiplex Microarrays: The Next Generation ELISA

The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2).  These autoantibodies are increasingly measured to guide clinical decision making.    The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued

U1 snRNP and Smith Antigens in Autoimmune Diagnostics

We are pleased to present a recent talk by Grace Bio-Labs Scientist Kevin Carman.       Summary: U1 snRNP and Smith antigen auto-antibodies are important markers in several autoimmune disorders. Auto-antibodies against these extractable nuclear antigens (ENA’s) are commonly used as clinical diagnostic criteria for Sjorgen’s Syndrome, SLE, and MCTD.

Enabling Cell-based Assays and Translation to Personalized Medicine

Cell-based assays have expanded in use, and reduced in sample requirements over the past decade, driven by technologies that have improved the culture and monitoring of cell functions on a miniature scale. One of our partners, Microstem Inc., based in San Diego, CA, is an example of an early stage reagent development company that approached … Continued

Sensitive Detection of Total Protein on RPPA using Fast Green

Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued

Antigen Discovery – Pioneer in High Throughput Biomarker Discovery

Antigen Discovery Inc.’s protein microarrays allow for highly multiplexed assays the generate hundreds, thousands and tens of thousands data points for each sample tested. Antigen Discovery Inc., our collaborators and our customers have leveraged this technology to peer into the complex intricacies of the immune response to disease generating over 80 peer-reviewed research articles in … Continued

A Systems Approach to Quantitative Multiplexed RPPA

A SYSTEMS APPROACH TO QUANTITATIVE MULTIPLEXED RPPA’S Presented by: Michael A. Shultz, PhD  RPPA Workshop in Kobe, Japan November, 2013 Porous nitrocellulose film (PNC) is the substrate of choice for RPPA largely because of its extraordinarily high protein binding capacity, which can exceed non-porous planar counterparts by 500X.

Beating Background Fluorescence on Nitrocellulose Film-Slides

Near Infrared Detection with Porous Nitrocellulose Films Improves Sensitivity for Protein Arrays. Despite some clear performance advantages of porous nitrocellulose Film-Slides for protein arrays, one objection to their use is the inherent fluorescence background detected on most scanners when using popular organic dyes with visible emission wavelengths (500-650 nm). However, fluorescence detection in near infrared … Continued