CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas-9 knockout of glucose 6-phosphate dehydrogenase (G6PD) The first surviving G6PD- knockout somatic cell line was generated with CRISPR-Cas-9 technology to support subcellular localization studies. G6PD, glucose 6-phosphate dehydrogenase, is the rate-limiting enzyme in the pentose phosphate pathway. G6PD is involved in oxidative stress, apoptosis, angiogenesis and cancer … Continued
Category: Featured Research
Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued
George Mason University Poster Presentation for the National Cancer Institute’s 15th Annual IMAT Principle Investigators Meeting, November 13-15, 2014. Unmet need: A significant and underappreciated issue is the fact that excised tissue is alive and reacting to ex vivo stress . During this “cold ischemia time” cells within the tissue react and adapt to the absence … Continued
The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2). These autoantibodies are increasingly measured to guide clinical decision making. The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued
We are pleased to present a recent talk by Grace Bio-Labs Scientist Kevin Carman. Summary: U1 snRNP and Smith antigen auto-antibodies are important markers in several autoimmune disorders. Auto-antibodies against these extractable nuclear antigens (ENA’s) are commonly used as clinical diagnostic criteria for Sjorgen’s Syndrome, SLE, and MCTD.
Cell-based assays have expanded in use, and reduced in sample requirements over the past decade, driven by technologies that have improved the culture and monitoring of cell functions on a miniature scale. One of our partners, Microstem Inc., based in San Diego, CA, is an example of an early stage reagent development company that approached … Continued
Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued
Antigen Discovery Inc.’s protein microarrays allow for highly multiplexed assays the generate hundreds, thousands and tens of thousands data points for each sample tested. Antigen Discovery Inc., our collaborators and our customers have leveraged this technology to peer into the complex intricacies of the immune response to disease generating over 80 peer-reviewed research articles in … Continued
A SYSTEMS APPROACH TO QUANTITATIVE MULTIPLEXED RPPA’S Presented by: Michael A. Shultz, PhD RPPA Workshop in Kobe, Japan November, 2013 Porous nitrocellulose film (PNC) is the substrate of choice for RPPA largely because of its extraordinarily high protein binding capacity, which can exceed non-porous planar counterparts by 500X.
Near Infrared Detection with Porous Nitrocellulose Films Improves Sensitivity for Protein Arrays. Despite some clear performance advantages of porous nitrocellulose Film-Slides for protein arrays, one objection to their use is the inherent fluorescence background detected on most scanners when using popular organic dyes with visible emission wavelengths (500-650 nm). However, fluorescence detection in near infrared … Continued
Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc. ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides. Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued
Blocking reagents are used to reduce non-specific protein binding in immunoassays, significantly improving results in terms of Signal-to-Noise ratio. Typically the blocking reagent is added prior to addition of the primary detection antibody.In this study we asked the question as to whether addition of the blocking reagent to other steps in a protein array assay … Continued