Category: Cell Culture

CS-16 CultureWell Chambered Coverglass Used To Validate A Reverse Genetic System For The Study of Orthobunyavirus

Orthobunyavirus is a genus of the Peribunyaviridae family in the order Bunyavirales, currently comprising ~170 recognized viruses. Most orthobunyavirus species are transmitted by gnats and cause diseases in animals or humans. Their genomic organization is such that reassortment with related viruses is possible, resulting in altered virulence and zoonotic potential. Shuni virus (SHUV) is an orthobunyavirus related to Schmallenberg virus (SBV) that causes neurological disease in … Continued

Expansion Microscopy

Expansion microscopy is a recently developed technique that allows nanoscale resolution imaging of specimens on fast, diffraction-limited microscopes. The method is based on the use of highly cross-linked, expandable polymers that bind biomolecules and labels. The expansion of the polymer pulls biomolecules and labels apart. As a result, molecules are moved away from each other … Continued

Confocal Fluorescence Microscopy of CRISPR-generated G6PD Knockout mouse liver cells (TIB-73)

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas-9 knockout of glucose 6-phosphate dehydrogenase (G6PD) The first surviving G6PD- knockout somatic cell line was generated with CRISPR-Cas-9 technology to support subcellular localization studies. G6PD,  glucose 6-phosphate dehydrogenase, is the rate-limiting enzyme in the pentose phosphate pathway. G6PD is involved in oxidative stress, apoptosis, angiogenesis and cancer … Continued