First, in an interesting Shistotosomiasis study, Gaze, et al, employ protein microarrays in a longitudinal immunomics study for vaccine antigen discovery (1). Schistosomiasis is a pervasive disease affecting 200 million people a year and traditional drug treatments are ineffective due to re-infection, drug expense, and parasite drug resistance. Non-sterile immunity does develop over long periods of … Continued
A comprehensive assessment of multiplex immunoassay platforms and detection methods is summarized in an interesting 2011 article by Chandra, et al, in Expert Review Proteomics (1). The article, “Protein Microarrays and Novel Detection Platforms” provides interesting information for several reasons.First, it presents an excellent description of the emerging discovery and clinical need for high throughput … Continued
Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued
The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2). These autoantibodies are increasingly measured to guide clinical decision making. The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued
Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued
A SYSTEMS APPROACH TO QUANTITATIVE MULTIPLEXED RPPA’S Presented by: Michael A. Shultz, PhD RPPA Workshop in Kobe, Japan November, 2013 Porous nitrocellulose film (PNC) is the substrate of choice for RPPA largely because of its extraordinarily high protein binding capacity, which can exceed non-porous planar counterparts by 500X.