RNA sequential probing of targets (SPOTs) is a newly developed method for mRNAs profiling, based on sequential Fluorescence In Situ Hybridization (seqFISH). SeqFIH is a technology that enables the identification of many RNA transcripts directly in single cells preserving spatial context. To demonstrate the method “mRNA, extracted from mouse cells, was captured on a locked nucleic acid … Continued
Author: Grace Bio
HybriWell™ hybridization chambers are the ideal tool for conserving precious or costly reagents in imaging experiments. HybriWells™ are inexpensive, disposable devices that create a chamber whose ports allow liquid exchange via microfluidic passive pumping , as demonstrated in this video: Seitz et, al. recently applied the method to develop and test fluorescence-based genetically-encoded biosensors to study … Continued
Protein glycosylation is a complex post-translational modification (PTM) that provides great proteomic diversity. It involves the attachment of sugars at specific amino acid residues. Asn (N-linked) or Ser/Thr (O-linked) being the residues most frequently glycosylated. Glycosylation is critical for physiological and pathological cellular functions and affects the bioavailability, efficacy, stability, and solubility of therapeutic glycoproteins. … Continued
Surface-enhanced Raman spectroscopy (SERS) technology is increasingly being adopted in biomedical analysis and imaging. A group of researchers at the University of Padova, Italy recently adopted the technology to capture and analyze tumor cells in peripheral blood. Cancer cells released from the primary tumor into the lymphatic system or in the bloodstream have the potential … Continued
Scientists at Tufts University and Harvard Medical School have developed a method to cryopreserve infectious Cryptosporidium oocysts. The results were published in Scientific Reports. Cryptosporidium is a microscopic parasite that causes the diarrheal disease cryptosporidiosis. While this parasite can be spread in many different ways water is the main source of transmission. Infants and immunocompromised adults are most at … Continued
Humoral immune response profiling is a valuable tool for the identification of potential targets for vaccine development. Immunogenicity studies in clinical trials are usually performed by ELISA assay. However, technical constraints limit the number of antigens that can be simultaneously analyzed. These limitations can be overcome by employing microarray technology, where antigenic reactivity can be … Continued
Human-induced pluripotent stem cell (iPSC) technology has taken the center stage in regenerative medicine research thanks to its enormous potential for personalized treatment and drug screening applications. Reprogramming somatic cells to generate iPSCs (induced pluripotent stem cells) is not only an easier and faster way to obtain stem cells, but is also devoid of the ethical … Continued
For instance, in a recent study by Hung-Chi Y. et al., researchers used ProPlates to develop a new assay to detect arsenic in the environment. Arsenic contamination is a widespread health concern as inorganic arsenite (As(III)) is highly stable in the environment and extremely toxic to humans. The maximum concentration in drinking water allowed by … Continued
August 6th, 2020 10am to 3pm Click here to connect with our representatives and learn more about Grace Bio-Labs products and services for life science research.
August 5th, 2020 10am to 3pm. Use this link to connect with our representatives.
Convalescent plasma is a potential therapeutic option for patients with coronavirus disease. The therapy is based on the administration of plasma containing antibodies against SARS-CoV-2 to help patients boost their responses to the coronavirus. Additionally, convalescent plasma can also be used for passive immunization with the potential to prevent viral infection. In both applications, determining … Continued
Orthobunyavirus is a genus of the Peribunyaviridae family in the order Bunyavirales, currently comprising ~170 recognized viruses. Most orthobunyavirus species are transmitted by gnats and cause diseases in animals or humans. Their genomic organization is such that reassortment with related viruses is possible, resulting in altered virulence and zoonotic potential. Shuni virus (SHUV) is an orthobunyavirus related to Schmallenberg virus (SBV) that causes neurological disease in … Continued