ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM
ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM was developed by Grace Bio-Labs as an affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.
The ArrayCAM® 400S Imager combines imaging capability for both colorimetric and multiple fluorescent labels into one instrument. This CMOS, camera-based imager, allows microarray data to be collected at 10 µm resolution in a fraction of the time required by conventional digitizing scanners. Typical image capture takes less than one minute per 25 x 75mm chip.
The high power, UV diode laser produces an excitation wavelength ideally suited to excite a full spectrum of Qdot® labeled detection reagents as well as Fast Green and SYPRO Ruby® stains commonly used to quantify total protein. Interchangeable filters onboard the instrument support a large number of multiplexing applications allowing investigators to measure several biomarkers, at the same time on a single spot within the same microarray without a loss of sensitivity.
User-friendly, software provides a full menu of features to optimize imaging and analysis. 16-bit files may be exported in a variety of formats including TIFF and PNG. Tools for automated image analysis produce data output from full-slide images, in seconds.
* Qdot® and SYPRO Ruby® are registered Trademarks of Life Technologies Inc.
ARRAYCAM® 400S IMAGER
ArrayCAM® 400S is a personal microarray imager designed for the rapid capture and analysis of microarrays printed onto ONCYTE nitrocellulose coated chips. This portable imager is ideally suited for the simultaneous detection of multiple biomarkers using several Qdot® labeled detection probes*, certain fluorescent dyes and precipitating colorimetric assay results. Software included with the instrument features a selectable menu of parameters to optimize image capture and analysis. Automatic mapping, preview and flagging of spots which do not conform to user selected criteria saves time and provides consistency in data output. For a complete description of features and how to use them please refer to the ArrayCAM® 400S Operation Manual * Qdot® is a registered Trademark of Life Technologies Inc.
Tutorial 1: Capture
Tutorial 2: Image Adjustments
Tutorial 3: GAL Files
Tutorial 4: Analysis Options
Taghavian, O., Jain, A., Joyner, C. J., Ketchum, S., Nakajima, R., Jasinskas, A., Liang, L., Fong, R., King, C., Greenhouse, B., Murphy, M., Bailey, J., Galinski, M. R., Barnwell, J. W., P., Christopher V., Davies, D. H., Felgner, P. L. "Antibody Profiling by Proteome Microarray with Multiplex Isotype Detection Reveals Overlap between Human and Aotus nancymaae Controlled Malaria Infections." PROTEOMICS, January 1, 2018. http://onlinelibrary.wiley.com/doi/10.1002/pmic.201700277/abstract
Kalantari-Dehaghi, Mina, Sookhee Chun, Aziz Alami Chentoufi, Jozelyn Pablo, Li Liang, Gargi Dasgupta, Douglas M. Molina, et al. “Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling.” Journal of Virology 86, no. 8 (April 2012): 4328–39. http://doi.org/10.1128/JVI.05194-11.
Q: What methods does the ArrayCAM software use to locate spots?
A: There are two different methods.
1. Fiducial template method
This method requires fiducials to be printed and developed. The fiducials will be located at the top of the microarray as the first row in the array. A template is created by drawing a rectangle around the fiducial row. Once the template is created, it can be saved and used for all microarrays that have the same configuration (print map). Batch Analysis uses this method.
2. Non-fiducial method
In this method, you need to place a location rectangle around the first row in the microarray (via the software). This will need to be repeated for each image to be analyzed.
Q: Do you need a gal file to perform spot analysis?
A: Yes. And you need to tag each spot in the gal file according to its type in column 6.
Q: What are the permitted spot tags?
A: The tags are Fiducial, Positive, Negative, Analyte, Buffer, Blank, and Control. The tags do not need to be capitalized, but they do need to be spelled correctly. The software will locate and analyze spots corresponding to any combination of the seven tags.
Q: What is the format of the results file?
A: Tab-delimited text with column headers.
Q: How big is the ArrayCAM?
A: Overall dimensions: 8.23in x 10.6in x 14.02in (WxDXH). Weight: 19.6 lb.
Q: How long does Batch Analysis take?
A: The software analyzes approximately 100 spots per second, so it depends on how many arrays are being analyzed and the number of spots in each array. An 8-pad slide with 1156 spots (17x68 array) on each pad takes about 1.5 minutes to analyze.
Q: How should the slide be oriented in the ArrayCAM for imaging?
A: When inserting a slide into the imager, the content needs to be facing down and the top of the slide needs to be to the right.
ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM
Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.
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