ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM was developed by Grace Bio-Labs as an affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.
The ArrayCAM® 400S Imager combines imaging capability for both colorimetric and multiple fluorescent labels into one instrument. This CMOS, camera-based imager, allows microarray data to be collected at 10 µm resolution in a fraction of the time required by conventional digitizing scanners. Typical image capture takes less than one minute per 25 x 75mm chip.
The high power, UV diode laser produces an excitation wavelength ideally suited to excite a full spectrum of Qdot® labeled detection reagents as well as Fast Green and SYPRO Ruby® stains commonly used to quantify total protein. Interchangeable filters onboard the instrument support a large number of multiplexing applications allowing investigators to measure several biomarkers, at the same time on a single spot within the same microarray without a loss of sensitivity.
User-friendly, software provides a full menu of features to optimize imaging and analysis. 16-bit files may be exported in a variety of formats including TIFF and PNG. Tools for automated image analysis produce data output from full-slide images, in seconds.
* Qdot® and SYPRO Ruby® are registered Trademarks of Life Technologies Inc.
ARRAYCAM® 400S IMAGER
ArrayCAM® 400S is a personal microarray imager designed for the rapid capture and analysis of microarrays printed onto ONCYTE nitrocellulose coated chips. This portable imager is ideally suited for the simultaneous detection of multiple biomarkers using several Qdot® labeled detection probes*, certain fluorescent dyes and precipitating colorimetric assay results. Software included with the instrument features a selectable menu of parameters to optimize image capture and analysis. Automatic mapping, preview and flagging of spots which do not conform to user selected criteria saves time and provides consistency in data output. For a complete description of features and how to use them please refer to the ArrayCAM® 400S Operation Manual * Qdot® is a registered Trademark of Life Technologies Inc.
Technical Resources
USER GUIDES
User Guide - ArrayCAM® Quick Start Guide
ArrayCAM® Single Slide Operation Manual
User Guide ArrayCAM® 400S Analysis using Fiducials
User Guide - ArrayCAM® 400S Analysis without using Fiducials
User Guide - ArrayCAM® 400S Batch Analysis
PROTOCOLS
Protocol - SYPRO Ruby® Staining of ONCYTE® Porours Nitrocellulose Film Slides
Protocol - Fast Green Staining of ONCYTE® Porous Nitrocellulose Film Slides with Near IR Detection
ArrayCAM® Multiplex Human Serology Assay Protocol
Videos
Tutorial 1: Capture
Tutorial 2: Image Adjustments
Tutorial 3: GAL Files
Tutorial 4: Analysis Options
References
Nakajima R, Supnet M, Jasinskas A, Jain A, Taghavian O, Obiero J, Milton DK, Chen WH, Grantham M, Webby R, Krammer F, Carter D, Felgner PL, Davies DH. "Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific Antibodies." mSphere. 2018: https://msphere.asm.org/content/3/6/e00592-18
Taghavian, O., Jain, A., Joyner, C. J., Ketchum, S., Nakajima, R., Jasinskas, A., Liang, L., Fong, R., King, C., Greenhouse, B., Murphy, M., Bailey, J., Galinski, M. R., Barnwell, J. W., P., Christopher V., Davies, D. H., Felgner, P. L. "Antibody Profiling by Proteome Microarray with Multiplex Isotype Detection Reveals Overlap between Human and Aotus nancymaae Controlled Malaria Infections." PROTEOMICS, January 1, 2018. http://onlinelibrary.wiley.com/doi/10.1002/pmic.201700277/abstract
Felgner J, Jain A, Nakajima R, Liang L, Jasinskas A, Gotuzzo E, Vinetz JM, Miyajima F, Pirmohamed M, Hassan-Hanga F, Umoru D, Jibir BW, Gambo S, Olateju K, Felgner PL, Obaro S, Davies DH. "Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children." PLoS Negl Trop Dis. 2017: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005679.
Charles RC, Nakajima R, Liang L, Jasinskas A, Berger A, Leung DT, Kelly M, Xu P, Kovác P, Giffen SR, Harbison JD, Chowdhury F, Khan AI, Calderwood SB, Bhuiyan TR, Harris JB, Felgner PL, Qadri F, Ryan ET."Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh." J Infect Dis. 2017. https://academic.oup.com/jid/article/216/1/125/3848946
Uplekar S, Rao PN, Ramanathapuram L, Awasthi V, Verma K, Sutton P, Ali SZ, Patel A, G SL, Ravishankaran S, Desai N, Tandel N, Choubey S, Barla P, Kanagaraj D, Eapen A, Pradhan K, Singh R, Jain A, Felgner PL, Davies DH, Carlton JM, Das J. Characterizing Antibody Responses to Plasmodium vivax and Plasmodium falciparum Antigens in India Using Genome-Scale Protein Microarrays. PLoS Negl Trop Dis. 2017: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005323
Jain A, Taghavian O, Vallejo D, Dotsey E, Schwartz D, Bell FG, Greef C, Davies DH, Grudzien J, Lee AP, Felgner PL, Liang L. Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays. Proteomics. 2016: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3318641/
Kalantari-Dehaghi, Mina, Sookhee Chun, Aziz Alami Chentoufi, Jozelyn Pablo, Li Liang, Gargi Dasgupta, Douglas M. Molina, et al. “Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling.” Journal of Virology 86, no. 8 (April 2012): 4328–39. http://doi.org/10.1128/JVI.05194-11.
FAQ
Q: What methods does the ArrayCAM software use to locate spots?
A: There are two different methods.
1. Fiducial template method
This method requires fiducials to be printed and developed. The fiducials will be located at the top of the microarray as the first row in the array. A template is created by drawing a rectangle around the fiducial row. Once the template is created, it can be saved and used for all microarrays that have the same configuration (print map). Batch Analysis uses this method.
2. Non-fiducial method
In this method, you need to place a location rectangle around the first row in the microarray (via the software). This will need to be repeated for each image to be analyzed.
Q: Do you need a gal file to perform spot analysis?
A: Yes. And you need to tag each spot in the gal file according to its type in column 6.
Q: What are the permitted spot tags?
A: The tags are Fiducial, Positive, Negative, Analyte, Buffer, Blank, and Control. The tags do not need to be capitalized, but they do need to be spelled correctly. The software will locate and analyze spots corresponding to any combination of the seven tags.
Q: What is the format of the results file?
A: Tab-delimited text with column headers.
Q: How big is the ArrayCAM?
A: Overall dimensions: 8.23in x 10.6in x 14.02in (WxDXH). Weight: 19.6 lb.
Q: How long does Batch Analysis take?
A: The software analyzes approximately 100 spots per second, so it depends on how many arrays are being analyzed and the number of spots in each array. An 8-pad slide with 1156 spots (17x68 array) on each pad takes about 1.5 minutes to analyze.
Q: How should the slide be oriented in the ArrayCAM for imaging?
A: When inserting a slide into the imager, the content needs to be facing down and the top of the slide needs to be to the right.
