Nitrocellulose-coated microarray substrates for protein, glycan and cell lysate arrays, tissue and cell printing and other applications where binding affinity, binding capacity and preservation of native bioactivities are important.

ONCYTE® Nitrocellulose Film Slides and Film Plates

The nitrocellulose Film-slide and Film-plate technology, trademarked ONCYTE®, was invented by Grace Bio-Labs scientists. The ONCYTE® product is a three dimensional microporous film cast on a variety of solid surfaces (vis. Glass, plastic) comprised of a combination of nitrocellulose polymer and proprietary chemistry that provides an ideal surface for marker discovery and validation as well as study of protein function. ONCYTE® nitrocellulose Films excel in various applications including:

Antibody/Protein Capture Microarrays (Knezevic et al, 2001; Scorilas et al, 2000)	Cell or Tissue Lysate Microarrays (Paweletz et al, 2001; Sheehan et al, 2005)
Lectin/Glycan Capture Microarrays (Fukui et al, 2002; Wang D et al, 2002)	Whole Proteome Microarrays (Davies et al, 2005a; Michaud et al, 2003)
Protein-Protein Interactions (Espejo et al, 2002; Schweitzer et al, 2003)	Protein-DNA Interactions (Kersten et al, 2004; Ho et al, 2006)
Protein Domain Microarrays (Espejo et al, 2002; MacBeath et al, 2002)	Cytocoherent Transfer (McGrath et al, 1991)
Peptide Microarrays (Michaud et al, 2003; Poetz et al, 2005)	Tissue Printing (Van Herpen et al, 2008; Wiley et al, 2007)

The performance advantages of ONCYTE® Film for each of these applications is crafted from chemistry manipulations.

Grace has built a strong set of Film Values with ONCYTE®

- 11 Film patterns available on glass or plastic microscope slides--see below

- Film chemistry is resistant to standard Reblot™ and antigen retrieval protocols

- Coating is uniformly flat and amenable to automated printing with contact or non-contact devices

- Can be coated on various surface chemistries (vis. Gold, Tantalum)

- Can be customized to any Film pattern

- Strong R&D support for new applications

- Strong quality support for variance control of key performance indicators

Grace Bio-Labs offers two distinct nitrocellulose film coatings for protein microarrays:

ONCYTE® AVID™ for applications that require a high binding capacity (vis. Reverse phase tissue arrays). Marker detection with colorimetric, luminescent, NIR or radioactive end-points is desirable with this Film. Fluorescent end-points can also be used with special blocking or quenching protocols (call for information).

ONCYTE® NOVA™ for applications that require low autofluorescence backgrounds.

Typical autofluorescence backgrounds and binding capacities for Avid™ and Nova™ Films are illustrated in the following graphs:

Background Fluorescence.jpg Protein Binding Nova Avid.jpg

Nitrocellulose Film Structure Provides Additional Assay Flexibility For Custom Applications

The parameters of nitration, pore size, pore density, pore shape and void volume all influence protein capture and detection sensitivities and can be systematically varied to meet specific demands. For example, pore structure can be changed from an “open weave”, which is preferred for “flow through” applications, to a “closed pore” which is more suitable for high resolution microscopic applications (McGrath et al, 1991).

Similarly, void volumes can be controlled to accommodate chromatographic or other transfer applications. Other chemistries can be added to Film; for example, chemistries to preserve phosphoproteins (Espina et al, 2008; 2009) or to lower autofluorescence backgrounds. Nitrocellulose can be derivatized to put functional groups on the backbone (Wang X et al, 2009) to augment or change molecular binding properties (Wang X et al, 2010).

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Ordering Information

We can easily customize pad size and placement for your application.

ITEM	CLICK ICON FOR FULL DIMENSIONS	DESCRIPTION	QUANTITY	PRICE
305170		ONCYTE® AVID 1- 20mm x 51mm NC pad per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$433.00
505170		ONCYTE® NOVA 1- 20mm x 51mm NC pad per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$433.00
305177		ONCYTE® AVID 1- 20mm x 60mm NC pad per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$495.00
505177		ONCYTE® NOVA 1- 20mm x 60mm NC pad per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$495.00
305278		ONCYTE® AVID 1- 21mm x 71mm NC pad per slide; Pad placement +/- 0.3 mm; Glass measures 25 x 75 x 1mm	20 Per Pack	$495.00
505278		ONCYTE® NOVA 1- 21mm x 71mm NC pad per slide; Pad placement +/- 0.3 mm; Glass measures 25 x 75 x 1mm	20 Per Pack	$495.00
305002		ONCYTE® AVID 2- 20mm x 20mm NC pads per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$536.00
505002		ONCYTE® NOVA 2- 20mm x 20mm NC pads per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$536.00
305008		ONCYTE® AVID 8- 6mm x 6mm NC pads per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$536.00
505008		ONCYTE® NOVA 8- 6mm x 6mm NC pads per slide; Frosted End Glass 25 x 75 x 1mm	20 Per Pack	$536.00
305012		ONCYTE® AVID 12- 6mm x 6mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$577.00
505012		ONCYTE® NOVA 12- 6mm x 6mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$577.00
305016		ONCYTE® AVID 16- 6mm x 6mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
505016		ONCYTE® NOVA 16- 6mm x 6mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
305116		ONCYTE® AVID 16- 6.5mm x 6.5mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
505116		ONCYTE® NOVA 16- 6.5mm x 6.5mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
305064		ONCYTE® AVID 64- 2.5mm x 2.5mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$721.00
505064		ONCYTE® NOVA 64- 2.5mm x 2.5mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$721.00
305101		ONCYTE® AVID 1- 15mm x 69mm NC pad per slide; Glass 25 x 75 x 1mm	20 Per Pack	$536.00
505101		ONCYTE® NOVA 1- 15mm x 69mm NC pad per slide; Glass 25 x 75 x 1mm	20 Per Pack	$536.00
305102		ONCYTE® AVID 2- 15mm x 32mm NC pads per slide; Glass 25 x 75 x 1mm	20 Per Pack	$536.00
505102		ONCYTE® NOVA 2- 15mm x 32mm NC pads per slide; Glass 25 x 75 x 1mm	20 Per Pack	$536.00
305004		ONCYTE® AVID 4- 15mm x 15mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$536.00
505004		ONCYTE® NOVA 4- 15mm x 15mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$536.00
305108		ONCYTE® AVID 8- 6mm x 15mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
505108		ONCYTE® NOVA 8- 6mm x 15mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$660.00
305118		ONCYTE® AVID 8- 6mm x 20mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$618.00
505118		ONCYTE® NOVA 8- 6mm x 20mm NC pads per slide; Plain Glass 25 x 75 x 1mm	20 Per Pack	$618.00
ONCYTE® For SBS Compliant Microtiter Plates
305096		ONCYTE® AVID 96 NC Round Pads 6mm diameter for SBS Microtiter Plate with Glass Substrate	Each	$175.00
505096		ONCYTE® NOVA 96 NC Round Pads 6mm diameter for SBS Microtiter Plate with Glass Substrate	Each	$175.00
305196		ONCYTE® AVID 96 NC Square Pads 6mm x 6mm for SBS Microtiter Plate with Glass Substrate	Each	$175.00
505196		ONCYTE® NOVA 96 NC Square Pads 6mm x 6mm for SBS Microtiter Plate with Glass Substrate	Each	$175.00
305384		ONCYTE® AVID 384 NC Square Pads 2.5mm x 2.5mm for SBS Microtiter Plate with Glass Substrate	Each	$199.00
505384		ONCYTE® NOVA 384 NC Square Pads 2.5mm x 2.5mm for SBS Microtiter Plate with Glass Substrate	Each	$199.00
ONCYTE® Barcode Formats
300001		ONCYTE® Barcode 20mm x 7.5mm; Code 128 Format	Each	$1.20
300002		ONCYTE® Barcode 20mm x 8.5mm; Code 128 Format	Each	$1.20
300003		ONCYTE® Barcode 22mm x 14mm; CODABAR Format	Each	$1.20